Effect Of Ph On Enzyme

Investigating The Effect of pH on Enzyme Activity
unveiling:
Enzymes are very sensitive to pH changes. Most enzymes bring in best at a pH of 7, but this is non always the case, for example, enzymes in the stomach work best optimally at very low pHs.
The pH at which an enzyme whole shebang best is described as its optimum pH.
At pHs ut near form the optimum pH, the enzyme can become denatured.
Aim of mulish: To find out the effect of pH on an enzyme reaction.
proposal:
Amylase is an enzyme that breaks bring starch to a sugar (maltose).
-Label 5 runnel tubes 1-5.
-Place 5cm3 of starch upshot into each test tube.
-Add 2cm3 of pH solution to each test tube. The pHs include pH3 (acid), pH6 (slightly acid), pH7 (neutral), pH8 (slightly alkaline) and pH9 (alkaline).
-Place 1cm3of amylase enzyme into each test tube. conjure up the tubes gently and immediately start stopclock.
-Every minute remove a sample and test with one/two drops of unity solution.
-Continue until iodine stays brown. Record sequence for the starch to be impoverished down in seconds in the table below.




Results Table:
pH| Time find outn for starch to be broken down by amylase (in seconds)|
3 (acid)| 900 |
5 (slightly acid)| 600|
7 (neutral)| 240|
8 (slightly alkaline)| 360|
9 (alkaline)| |

Questions
a) P accord a graph of pH on the x-axis and time taken for starch to be broken down (in seconds) on the y-axis.
b) At what pH did the enzyme, amylase, work most rapidly? Explain
* The amylase worked most rapidly at pH7. This is because the Starch enzyme would work best at ph7 as that is our bodys standard pH.
c) Your stomach pH is about 2. Would you expect starch digestion to take place in the stomach?
* I would not expect Starch digesting to take place in the stomach and it would take a lot of time to digest.If you want to get a full essay, range it on our website: Orderessay



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